3INU
Crystal structure of an unbound KZ52 neutralizing anti-Ebolavirus antibody.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 129.520, 129.520, 184.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.955 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n0x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.202 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.6D) |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.118 | 0.627 |
| Total number of observations | 42658 | |
| Number of reflections | 114050 | |
| <I/σ(I)> | 7.5 | 2 |
| Completeness [%] | 99.0 | 98.8 |
| Redundancy | 6.99 | 6.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 1.5 M ammonium sulfate, 0.1 M Tris-HCl pH 8.5 and 12% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
