3ING

Crystal structure of Homoserine dehydrogenase (NP_394635.1) from THERMOPLASMA ACIDOPHILUM at 1.95 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97927,0.97908
Spacegroup name	C 2 2 21
Unit cell lengths	69.821, 92.113, 120.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.105 - 1.950
R-factor	0.154
Rwork	0.152
R-free	0.19300
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.623
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.105	28.105	2.020
High resolution limit [Å]	1.950	4.190	1.950
Rmerge	0.079	0.026	0.499
Number of reflections	28686	5418	5339
<I/σ(I)>	8.45	24.8	1.6
Completeness [%]	98.6	97.8	97.2
Redundancy	3.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.14	293	49.0000% 2-propanol, 5.0000% polyethylene glycol 1000, 0.1M citric acid pH 5.14, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K