3IMQ
Crystal structure of the NusB101-S10(delta loop) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9788 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 112.630, 112.630, 263.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.20693 |
| Rwork | 0.204 |
| R-free | 0.25582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d3b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 29761 | |
| <I/σ(I)> | 18.1 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M potassium citrate, 20 % PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
