3IMK

Crystal structure of Putative molybdenum carrier protein (YP_461806.1) from SYNTROPHUS ACIDITROPHICUS SB at 1.45 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97936,0.97922
Spacegroup name	P 21 21 21
Unit cell lengths	41.250, 59.255, 64.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.479 - 1.450
R-factor	0.154
Rwork	0.152
R-free	0.17700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.515
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.479	28.479	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.050	0.027	0.593
Number of reflections	28613	5419	4895
<I/σ(I)>	15.2	39.7	2.3
Completeness [%]	98.4	98.5	93.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	10.0000% Glycerol, 5.0000% PEG-1000, 30.0000% PEG-600, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K