3IMB
Alternative binding mode of restriction endonuclease BcnI to cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.562, 84.053, 98.947 |
| Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.890 |
| R-factor | 0.21405 |
| Rwork | 0.212 |
| R-free | 0.25101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2odi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.089 | 0.236 |
| Number of reflections | 98933 | |
| <I/σ(I)> | 5.4 | 3.1 |
| Completeness [%] | 98.4 | 99.6 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.16M Ammonium Sulfate, 0.08M Sodium Acetate trihydrate pH 4.6, 20% w/v Polyethylene Glycol 4000, 20% v/v Glycerol Anhydrous, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
