3IM7
Crystal structure of mouse Ryanodine Receptor 2 N-terminal domain (1-217) disease mutant A77V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.211, 112.211, 36.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.310 - 2.210 |
| R-factor | 0.22497 |
| Rwork | 0.223 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3im6 RyR2 NTD V186M mutant |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.310 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 10981 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 93.3 | 59.8 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.2M sodium malonate, 5-15% saturated ammonium sulphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
