3IM3
Crystal structure of PKA RI alpha dimerization/docking domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2008-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 44.122, 44.122, 93.036 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.081 - 2.000 |
R-factor | 0.193 |
Rwork | 0.187 |
R-free | 0.24880 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.835 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 4047 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 36.9 | 27.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 9 | 298 | 30% PEG 3350, 0.2 mM Sodium formate, 0.1 M Bis-Tris propane pH 9.0, MICROBATCH, temperature 298K |