3IM1

Structure of the C-terminal Sec63 unit of yeast Brr2, P212121 Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.05
Spacegroup name	P 21 21 21
Unit cell lengths	55.235, 76.504, 84.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.19507
Rwork	0.193
R-free	0.23159
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.238
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.650	1.650
Number of reflections	43474
<I/σ(I)>	30.5	2.1
Completeness [%]	99.0	99.7
Redundancy	4.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100mM sodium cacodylate, pH 6.0, 100mM Li2SO4, 15% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K