3IM0

Crystal structure of Chlorella virus vAL-1 soaked in 200mM D-glucuronic acid, 10% PEG-3350, and 200mM glycine-NaOH (pH 10.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	40.402, 71.121, 100.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.660
R-factor	0.1634
Rwork	0.161
R-free	0.21630
Structure solution method	AB INITIO
Starting model (for MR)	3gne
RMSD bond length	0.009
RMSD bond angle	0.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.320	1.720
High resolution limit [Å]	1.660	1.660
Rmerge	0.040	0.144
Number of reflections	34464
<I/σ(I)>	74.6	16.5
Completeness [%]	97.5	97.5
Redundancy	5.4	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	12% PEG 3350, 0.2M ammonium formate, 0.1M hepes-NaOH pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K