3ILW
Structure of DNA gyrase subunit A N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.228, 70.054, 97.812 |
| Unit cell angles | 109.96, 91.16, 114.16 |
Refinement procedure
| Resolution | 45.160 - 1.603 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ab4 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.562 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (2009_02_15_2320_3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.478 |
| Number of reflections | 154988 | |
| <I/σ(I)> | 13.3 | 1.95 |
| Completeness [%] | 86.5 | 43.5 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 26% 2-methyl-2,4-pentanediol, 100mM Tris 9.0, 1mM Ciprofloxacin, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
