3ILV
Crystal structure of a glutamine-dependent NAD(+) synthetase from Cytophaga hutchinsonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 133.136, 66.345, 74.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.740 - 1.790 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.740 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.082 | 0.639 |
| Number of reflections | 57695 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 96.8 | 88.7 |
| Redundancy | 12.1 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M Sodium acetate trihydrate, 2.0M Sodium formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
