3ILM
Crystal Structure of the Alr3790 protein from Anabaena sp. Northeast Structural Genomics Consortium Target NsR437h
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.694, 77.293, 156.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.195 - 2.262 |
| R-factor | 0.2327 |
| Rwork | 0.232 |
| R-free | 0.24520 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.126 | 0.574 |
| Number of reflections | 46546 | |
| <I/σ(I)> | 19.4 | 2.4 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 7.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under parafin oil | 6 | 277 | 20% PEG 1000, 0.1M manganese chloride, 0.1M MES, pH 6.0, microbatch under parafin oil, temperature 277K |
