3ILK
The structure of a probable methylase family protein from Haemophilus influenzae Rd KW20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.167, 94.392, 124.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.560 - 2.010 |
R-factor | 0.20728 |
Rwork | 0.205 |
R-free | 0.24596 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.591 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.092 | 0.841 |
Number of reflections | 36940 | |
<I/σ(I)> | 25.9 | 1.4 |
Completeness [%] | 96.6 | 76 |
Redundancy | 5.1 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES, 10%w/v PEG8000, 8%v/v Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |