3ILE
Crystal structure of ORF157-E86A of Acidianus filamentous virus 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.876, 63.876, 85.352 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.000 - 3.300 |
| R-factor | 0.2582 |
| Rwork | 0.255 |
| R-free | 0.31281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ii2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.086 | 0.407 |
| Number of reflections | 3286 | |
| <I/σ(I)> | 21.2 | 5.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1M LiCl2, 0.01M NiCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
