3ILA
Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.734, 155.705, 81.613 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 48.510 - 2.900 |
| R-factor | 0.27653 |
| Rwork | 0.274 |
| R-free | 0.31991 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ryanodine Receptor isoform 2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.784 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 32354 | |
| <I/σ(I)> | 13.28 | 2.33 |
| Completeness [%] | 69.8 | 45.1 |
| Redundancy | 7.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
