3IL3
Structure of Haemophilus influenzae FabH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 68.196, 68.196, 277.737 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.900 - 2.700 |
| R-factor | 0.231 |
| Rwork | 0.223 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3il9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.040 | 0.175 |
| Number of reflections | 10562 | |
| <I/σ(I)> | 32.6 | 8.7 |
| Completeness [%] | 92.4 | 71.4 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | 35% PEG 4000, 0.4 M ammonium acetate, 0.1 M sodium citrate, 15% glycerol, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
