3IKR
Crystal structure of alpha 1-4 mannobiose bound trimeric human lung surfactant protein D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-24 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.520, 108.450, 55.820 |
| Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
| Resolution | 39.790 - 1.650 |
| Rwork | 0.193 |
| R-free | 0.21200 |
| Structure solution method | isomorphous to native crystals |
| Starting model (for MR) | 1pw9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.037 | 0.151 |
| Number of reflections | 74723 | |
| <I/σ(I)> | 19.6 | 7.6 |
| Completeness [%] | 94.5 | 87.7 |
| Redundancy | 4.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 4000, tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
