3IKQ
Crystal structure of alpha 1-2 mannobiose bound trimeric human lung surfactant protein D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-19 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.910, 108.780, 56.080 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 30.450 - 2.250 |
| Rwork | 0.202 |
| R-free | 0.23900 |
| Structure solution method | isomorphous to native crystals |
| Starting model (for MR) | 1pw9 |
| Data reduction software | MOSFLM (Version 6.2.3) |
| Data scaling software | SCALA (CCP4_3.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.450 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.059 | 0.222 |
| Number of reflections | 31524 | |
| <I/σ(I)> | 13.2 | 4.1 |
| Completeness [%] | 99.0 | 97.4 |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 4000, tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
