3IKK
Crystal structure analysis of msp domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-02 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 144.024, 144.024, 34.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.576 - 2.500 |
| R-factor | 0.2129 |
| Rwork | 0.207 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z9l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.302 | 0.895 |
| Number of reflections | 9305 | |
| <I/σ(I)> | 8.64 | 1.29 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.41 | 14.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200mM Ammonium acetate, 25% PEG3350, 0.1M Tril-HCl, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
