3IIU
Structure of the reconstituted Peridinin-Chlorophyll a-Protein (RFPCP) mutant N89L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.510, 81.730, 75.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.070 - 1.450 |
| R-factor | 0.15566 |
| Rwork | 0.155 |
| R-free | 0.17648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3IIS RFPCP |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.295 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.070 | 1.550 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 37771 | |
| <I/σ(I)> | 16.4 | 4.99 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.9 | 11.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 0.1M CdCl2, 0,1M Sodium Acetate pH 4.6, 20-24% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
