3IIR
Crystal Structure of Miraculin like protein from seeds of Murraya koenigii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.795, 75.795, 150.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.21985 |
| Rwork | 0.216 |
| R-free | 0.29452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ava |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.668 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.146 | 0.422 |
| Total number of observations | 58581 | |
| Number of reflections | 10300 | |
| <I/σ(I)> | 4.7 | 4.7 |
| Completeness [%] | 96.1 | 99.9 |
| Redundancy | 5.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 6% PEG 8000, 0.1mM Tris-Cl pH 8.0, 0.08M Sodium Chloride, 0.08M Calcium Chloride, 8% Glycerol , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
