3IIN
Plasticity of the kink turn structural motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 109.826, 109.826, 250.218 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.180 |
| R-factor | 0.292 |
| Rwork | 0.290 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6b |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.902 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.350 |
| High resolution limit [Å] | 4.180 | 9.030 | 4.180 |
| Rmerge | 0.101 | 0.036 | 0.789 |
| Number of reflections | 11924 | ||
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 99.4 | 95.9 | 100 |
| Redundancy | 6.3 | 5.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | MPD, Sodium Cacodylate, Magnesium Acetate, Potassium Acetate, Cobalt Hexamine, pH 6.8, vapor diffusion, hanging drop, temperature 298K |
