3II1
Structural characterization of difunctional glucanase-xylanse CelM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.594, 87.431, 110.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.640 - 2.250 |
| R-factor | 0.17295 |
| Rwork | 0.170 |
| R-free | 0.22131 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fw6 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Total number of observations | 147671 | |
| Number of reflections | 37193 | |
| Completeness [%] | 91.7 | 60 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 295.5 | 0.1M MES, pH 5.9, 10% (w/v) PEG 3350, 2mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
