3IHW

Crystal structure of the Ras-like domain of CENTG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-24
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	42.345, 54.176, 73.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 1.920
R-factor	0.233
Rwork	0.230
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bmj
RMSD bond length	0.016
RMSD bond angle	1.498
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.990
High resolution limit [Å]	1.920	4.130	1.920
Rmerge	0.138	0.043	0.990
Number of reflections	13556
<I/σ(I)>	6.6
Completeness [%]	100.0	100	100
Redundancy	6.8	6.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	16% PEG3350, 0.2M potassium fluoride, 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K