3IHU

Crystal structure of DNA binding protein (YP_298823.1) from Ralstonia eutropha JMP134 at 1.92 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97862,0.97799
Spacegroup name	C 1 2 1
Unit cell lengths	54.646, 79.135, 103.244
Unit cell angles	90.00, 102.08, 90.00

Refinement procedure

Resolution	29.361 - 1.920
R-factor	0.201
Rwork	0.199
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.489
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.361	29.360	1.970
High resolution limit [Å]	1.920	8.590	1.920
Rmerge	0.052	0.041	0.556
Total number of observations		709	6558
Number of reflections	32709
<I/σ(I)>	12.1	15.4	1.4
Completeness [%]	99.6	74.7	100
Redundancy	2.7	2.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.17	277	24.0000% polyethylene glycol 6000, 1.0000M lithium chloride, 0.1M TRIS pH 8.17, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K