3IHS

Crystal Structure of a Phosphocarrier Protein HPr from Bacillus anthracis str. Ames

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-14
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 31 2 1
Unit cell lengths	40.840, 40.840, 139.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.200 - 1.150
R-factor	0.15716
Rwork	0.156
R-free	0.18499
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.667
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.200	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.043	0.416
<I/σ(I)>	17.12	2.62
Completeness [%]	96.8	93.7
Redundancy	4.3	3.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 3350 20%, K dihydrogen Phosphate 0.2M, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 3350 20%, K dihydrogen Phosphate 0.2M, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K