3IHA
Crystal Structure Analysis of Mglu in its glutamate form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-10-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.451, 141.615, 75.149 |
| Unit cell angles | 90.00, 103.95, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.600 |
| Rwork | 0.271 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ih8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.380 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.119 | 0.356 |
| Number of reflections | 35920 | |
| <I/σ(I)> | 3.7 | 1.8 |
| Completeness [%] | 98.2 | 99.6 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15% PEG 4000, 100mM Sodium Acetate, 50mM HEPES, 200mM Glutamate, pH 7.5, vapor diffusion, hanging drop, temperature 293K |
