3IH9
Crystal Structure Analysis of Mglu in its tris form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-10-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.701, 141.393, 75.234 |
| Unit cell angles | 90.00, 104.48, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.500 |
| Rwork | 0.217 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ih8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.622 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.124 | 0.492 |
| Total number of observations | 149207 | |
| Number of reflections | 40451 | |
| <I/σ(I)> | 3.9 | 1.4 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 4000, 100mM Sodium Acetate, 50mM HEPES, 300mM Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
