3IH8
Crystal Structure Analysis of Mglu in its native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-05-17 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.076, 142.251, 74.248 |
| Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.300 |
| Rwork | 0.247 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3if5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.294 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.137 | 62.140 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.111 | 0.061 | 0.546 |
| Total number of observations | 189811 | 5710 | 27227 |
| Number of reflections | 52671 | ||
| <I/σ(I)> | 10.9 | 8.8 | 1.4 |
| Completeness [%] | 100.0 | 99.7 | 99.9 |
| Redundancy | 3.6 | 3.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15% PEG 4000, 100mM Sodium Acetate, 50mM HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 293K |
