3IH5
Crystal Structure of Electron Transfer Flavoprotein alpha-subunit from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 102.099, 102.099, 209.169 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.876 - 2.600 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.084 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.131 | 0.750 |
| Number of reflections | 39674 | |
| <I/σ(I)> | 8.2 | 3.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.7 M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
