3IGO
Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.388, 55.550, 81.697 |
| Unit cell angles | 90.00, 105.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dxn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.089 | 0.878 |
| Number of reflections | 25110 | |
| <I/σ(I)> | 7.3 | 1.65 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 3350, 0.2 M Diammonium tartrate, 2 mM ANP, CaCl2, TCEP, 4 mM MgCl2, 30% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
