3IG5
Saccharomyces cerevisiae glutamate cysteine ligase in complex with Mg2+ and L-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 117.444, 117.444, 165.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.910 - 2.100 |
R-factor | 0.1835 |
Rwork | 0.182 |
R-free | 0.21894 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.683 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.069 | 0.578 |
Number of reflections | 66287 | |
<I/σ(I)> | 20.9 | 1.8 |
Completeness [%] | 97.5 | 96.1 |
Redundancy | 4.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 12% PEG 400, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K |