3IFX
Crystal structure of the Spin-labeled KcsA mutant V48R1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.970, 76.630, 112.970 |
| Unit cell angles | 90.00, 125.83, 90.00 |
Refinement procedure
| Resolution | 24.840 - 3.560 |
| R-factor | 0.27267 |
| Rwork | 0.271 |
| R-free | 0.30218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bl8 |
| RMSD bond length | 0.055 |
| RMSD bond angle | 1.779 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0051) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.650 |
| High resolution limit [Å] | 3.560 | 3.560 |
| Rmerge | 0.086 | 0.042 |
| Number of reflections | 7898 | |
| <I/σ(I)> | 4 | 1.7 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 200 mM CaCl2, 150 mM KCl, 100 mM HEPES, 19-49% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
