3IFU
The Crystal Structure of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Leader Protease Nsp1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-05 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 1.2821, 1.2827 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 51.000, 51.000, 154.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.420 |
| R-factor | 0.231 |
| Rwork | 0.223 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.065 | 0.419 |
| Number of reflections | 8279 | |
| Completeness [%] | 99.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris-HCl, 25%(v/v) tert-butanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
