3IFP

X-ray structure of amyloid beta peptide:antibody (Abeta1-7:12B4) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 1
Unit cell lengths	78.860, 79.240, 94.100
Unit cell angles	68.66, 65.28, 78.47

Refinement procedure

Resolution	81.130 - 2.950
R-factor	0.2365
Rwork	0.235
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.157
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	81.130
High resolution limit [Å]	2.950
Rmerge	0.129
Number of reflections	38904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	Protein: 4.1 mg/ml, 10 mM Hepes pH 7.5, 75 mM NaCl. Protein:peptide molar ratio: 1:1.8. Reservoir: 30%PEG8K, 0.1M Hepes pH 7.0, 0.2M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 295K