3IFO

X-ray structure of amyloid beta peptide:antibody (Abeta1-7:10D5) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.07000
Spacegroup name	P 21 21 21
Unit cell lengths	96.310, 99.970, 103.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.383 - 2.150
R-factor	0.1651
Rwork	0.162
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.167
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.383
High resolution limit [Å]	2.150
Rmerge	0.058
Number of reflections	54491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Protein: 15 mg/ml, 10 mM Hepes pH 7.5, 75 mM NaCl. Protein:peptide molar ratio: 1:2. Reservoir: 30% PEG4K, VAPOR DIFFUSION, SITTING DROP, temperature 295K