3IF5

Crystal Structure Analysis of Mglu

Replaces:  2DFW

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791, 0.9793, 0.9700
Spacegroup name	F 2 2 2
Unit cell lengths	115.331, 116.680, 144.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.180 - 2.440
Rwork	0.204
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.300
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.180
High resolution limit [Å]	2.400
Number of reflections	17155
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	700mM Sodium Citrate, 50mM HEPES, 5mM MgCl2, 5% Glycerol, pH 7.5, vapor diffusion, hanging drop, temperature 298K