3IF0
Crystal Structure of the Nanoarchaeum equitans tRNA splicing endonuclease structural subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.434, 95.434, 47.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.380 - 2.200 |
| R-factor | 0.24964 |
| Rwork | 0.248 |
| R-free | 0.27712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iey |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.266 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.097 | 0.624 |
| Number of reflections | 13062 | |
| <I/σ(I)> | 10.5 | 4.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 19 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 303 | 0.10 M sodium acetate pH 4.6, 0.30 M ammonium acetate, 35% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
