3IEZ

Crystal structure of the RasGAP C-terminal (RGC) domain of IQGAP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	45.083, 47.256, 46.661
Unit cell angles	90.00, 95.51, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.209
Rwork	0.207
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.590
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (refmac_5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.042	0.025	0.239
Number of reflections	31015
<I/σ(I)>	17.3
Completeness [%]	98.8	99.9	87.5
Redundancy	5.7	7.2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	30% PEG-MME, 0.2M ammonium sulfate, 0.1M MES, 1:100 (w/w) chymotrypsin, pH 6.5, vapor diffusion, sitting drop, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	30% PEG-MME, 0.2M ammonium sulfate, 0.1M MES, 1:100 (w/w) chymotrypsin, pH 6.5, vapor diffusion, sitting drop, temperature 291K