3IEY
Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-08 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 |
| Unit cell lengths | 124.102, 124.102, 69.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.900 - 2.110 |
| R-factor | 0.23446 |
| Rwork | 0.232 |
| R-free | 0.25874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a79 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.064 | 0.305 |
| Number of reflections | 21470 | |
| <I/σ(I)> | 44.2 | 3.86 |
| Completeness [%] | 81.6 | 81.6 |
| Redundancy | 5.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 44% Tacsimate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
