3IEU
Crystal Structure of ERA in Complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.600, 67.900, 87.100 |
| Unit cell angles | 90.00, 114.20, 90.00 |
Refinement procedure
| Resolution | 24.687 - 2.800 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ega |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.068 | 0.717 |
| Number of reflections | 22917 | |
| <I/σ(I)> | 28.2 | 3.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 1.5 M Lithium Sulfate, 0.1 M Na Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
