3IE4
b-glucan binding domain of Drosophila GNBP3 defines a novel family of pattern recognition receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.530, 30.680, 51.650 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 29.850 - 1.450 |
| R-factor | 0.16686 |
| Rwork | 0.165 |
| R-free | 0.19811 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 1.520 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.067 | 0.296 |
| Number of reflections | 36216 | |
| <I/σ(I)> | 12.2 | 4.1 |
| Completeness [%] | 98.8 | 95.6 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M zinc acetate dehydrate, 18% PEG 8000, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
