3IDW

Crystal structure of Sla1 homology domain 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000,0.9796
Spacegroup name	P 65
Unit cell lengths	59.273, 59.273, 47.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.636 - 1.850
R-factor	0.204
Rwork	0.203
R-free	0.21900
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.218
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM (6.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.061	0.033	0.506
Number of reflections	8256
<I/σ(I)>	41.2
Completeness [%]	100.0	99.9	100
Redundancy	18.3	18.7	15.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	277	0.1 M HEPES, 5% PEG-6000, 5% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K