3IDQ
Crystal structure of S. cerevisiae Get3 at 3.7 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.320, 115.320, 281.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.054 - 3.701 |
| R-factor | 0.285 |
| Rwork | 0.283 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ibg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.147 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.4_62) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.054 | 3.900 |
| High resolution limit [Å] | 3.701 | 3.700 |
| Number of reflections | 7954 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M HEPES, 1.6 M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
