3ICX
Crystal structure of Sulfolobus solfataricus Nop5 (135-380)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.645, 122.126, 71.786 |
| Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.263 |
| Rwork | 0.261 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nnw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.154 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.149 | 0.475 |
| Number of reflections | 22817 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.8M (NH4)2SO4, 10 mM MgCl2, and 50mM Na cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
