3ICP

Crystal Structure of UDP-galactose 4-epimerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-07-06
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	101.055, 101.055, 75.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.550 - 2.010
R-factor	0.205
Rwork	0.204
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ehe
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.429	2.080
High resolution limit [Å]	2.010	2.010
Number of reflections	26762
<I/σ(I)>	15.8	9.8
Completeness [%]	100.0	100
Redundancy	14.1	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop	6.6	293	2-ethoxy ethanol, pH 6.6, sitting drop, temperature 293K