3ICA
The crystal structure of the beta subunit of a phenylalanyl-tRNA synthetase from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.725, 59.725, 256.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.440 |
| R-factor | 0.20505 |
| Rwork | 0.202 |
| R-free | 0.25486 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.088 | 0.497 |
| Number of reflections | 18220 | |
| <I/σ(I)> | 32.553 | 4.214 |
| Completeness [%] | 99.4 | 93.3 |
| Redundancy | 7.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M MgCl2, 0.1M Tris pH8.5, 30% v/v PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
