3IC8
The Crystal Structure of a GST-like protein from Pseudomonas syringae to 2.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.671, 102.266, 148.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.340 - 2.400 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.269 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (refmac_5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.092 | 0.662 |
| Number of reflections | 61123 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 20% PEG 3350, 0.2M Succinic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
