3IBX
Crystal structure of F47Y variant of TenA (HP1287) from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 148.728, 148.728, 233.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.000 - 2.400 |
| R-factor | 0.21831 |
| Rwork | 0.218 |
| R-free | 0.22957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rd3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.153 | 0.460 |
| Number of reflections | 51200 | |
| <I/σ(I)> | 8.6 | 3.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris, 1.1M lithium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
