3IBW
Crystal Structure of the ACT domain from GTP pyrophosphokinase of Chlorobium tepidum. Northeast Structural Genomics Consortium Target CtR148A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.306, 64.306, 93.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.930 - 1.930 |
| Rwork | 0.198 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.074 | 0.290 |
| Number of reflections | 28229 | |
| <I/σ(I)> | 34.9 | 9.5 |
| Completeness [%] | 99.7 | 96.9 |
| Redundancy | 8.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 8000, 0.17 ammonium phosphate, 0.085M cacodylic acid, 15% glycerol, pH 6.5 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
